Fatty Acid Solubilizer from the Oral Disk of the Blowfly
نویسندگان
چکیده
BACKGROUND Blowflies are economic pests of the wool industry and potential vectors for epidemics. The establishment of a pesticide-free, environmentally friendly blowfly control strategy is necessary. Blowflies must feed on meat in order to initiate the cascade of events that are involved in reproduction including juvenile hormone synthesis, vitellogenesis, and mating. During feeding blowflies regurgitate salivary lipase, which may play a role in releasing fatty acids from triglycerides that are found in food. However, long-chain fatty acids show low solubility in aqueous solutions. In order to solubilize and ingest the released hydrophobic fatty acids, the blowflies must use a solubilizer. METHODOLOGY We applied native PAGE, Edman degradation, cDNA cloning, and RT-PCR to characterize a protein that accumulated in the oral disk of the black blowfly, Phormia regina. In situ hybridization was carried out to localize the expression at the cellular level. A fluorescence competitive binding assay was used to identify potential ligands of this protein. CONCLUSION A protein newly identified from P. regina (PregOBP56a) belonged to the classic odorant-binding protein (OBP) family. This gene was expressed in a cluster of cells that was localized between pseudotracheae on the oral disk, which are not accessory cells of the taste peg chemosensory sensilla that normally synthesize OBPs. At pH 7 and pH 6, PregOBP56a bound palmitic, stearic, oleic, and linoleic acids, that are mainly found in chicken meat. The binding affinity of PregOBP56a decreased at pH 5. We propose that PregOBP56a is a protein that solubilizes fatty acids during feeding and subsequently helps to deliver the fatty acids to the midgut where it may help in the process of reproduction. As such, PregOBP56a is a potential molecular target for controlling the blowfly.
منابع مشابه
Evaluation of Changes in Fatty Acid Composition in Three Different Varieties of Olives During the Course of Maturation
ABSTRACT: Olives belong to the family of olea europaea L. is a popular fruit tree. The fruit and the oil extracted from it is consumed worldwide particularly in the Mediterranean regions. In this study changes in the fatty acid composition of the oil extracted from olive fruit during the course of maturation is investigated. This work is concerned with the best harvesting point when oleic acid ...
متن کاملSeasonal variations of total lipids, fatty acid composition, of Nizimuddinia zanardini from the seashore of Chabahar
The Iran’s coastal marine algae have great potential in the fields of medicine, food and cosmetics which unfortunately, fewer have been studied. In the present study, Total lipid and fatty acid profile of brown algae, N. zanardini were measured in both cold and warm season. The total lipid content with 41.1±0.8 mg/g dry weight was high in February. In the present study, Fatty acid composition o...
متن کاملSeasonal variations of ω3 polyunsaturated fatty acids in muscle of Capoeta caelestis
In this study, seasonal variations of fatty acid amounts in muscle tissues of Capoeta caelestis were investigated. We have found that the ω3 contents of C. caelestis in spring, summer, autumn and winter were 17.43%, 15.29%, 17.54%, 13.63% in female and 16.96%, 19.25%, 18.66%, 14.76% in male, respectively. The amounts of ω3 polyunsaturated fatty acids were higher than those of ω6 polyunsaturated...
متن کاملFatty acid composition of tench (Tinca tinca L., 1758): A seasonal differentiation
Seasonal variations of proximate compositions and fatty acids of tench (Tinca tinca) captured from Seyhan Dam Lake were investigated. The results showed that seasonal fatty acid compositions of tench fillets in July, August and November ranged from 29.59% to 33.71% for saturated fatty acids (SFA), from 20.50% to 21.69% for monounsaturated fatty acids (MUFAs) and from 26.50% to 41.85% for polyun...
متن کامل